The Ayurvedic Medicine Salacia oblonga Attenuates Diabetic Renal Fibrosis in Rats: Suppression of Angiotensin II/AT1 Signaling
Figure 6
Expression of collagen (Col) I (a), Col IV (b), fibronectin (c) mRNAs and fibronectin protein (d) in rat mesangial cells. SO (SO10: 10; SO20: 20 and SO40: 40 μg/ml), MA (25 μM) or telmisartan (Tel. 10 μM) was added 1 h before mesangial cells were treated with angiotensin II (10−6 M). Twenty-four hours later total RNA was extracted from the mesangial cells using TRIzol. The relative levels of specific mRNAs were determined by RT-PCR. Results were normalized to β-actin. In a parallel experiment, the medium was collected after mesangial cells were treated with the test agents in combination with angiotensin II. fibronectin protein was determined by commercial ELISA kit according to the manufacturer's instructions. All values are means ± SEM (n = 3, each group). *.
