The Ayurvedic Medicine Salacia oblonga Attenuates Diabetic Renal Fibrosis in Rats: Suppression of Angiotensin II/AT1 Signaling

Table 1

Sequences of primers used in the amplification of renal genes by RT-PCR

Gene	Accession no. or reference	Forward primer	Reverse primer	Size (bp)	Temp. (°C)
Collagen I	Li et al. [27]	5′-CATAAAGGGTCATCGTGGCTTC-3′	5′-GTGATAGGTGATGTTCTGGGAG-3′	~500	62
Collagen IV	Li et al. [27]	5′-GCAGGTGTGCGGTTTGTGAAG-3′	5′-AGCTCCCCTGCCTTCAAGGTG-3′	328	55
Fibronectin	NM019143	5′-CAAGACCATACCTGCCGAAT-3′	5′-CCGTGTAAGGGTCAAAGCAT-3′	417	63
AT1	NM030985	5′-GAGAGGATTCGTGGCTTGAG-3′	5′-TAAGTCAGCCAAGGCGAGAT-3′	464	62
TGF-β1	NM021578	5′-GACCTGCTGGCAATAGCTTC-3′	5′-GACTGGCGAGCCTTAGTTTG-3′	468	58
PAI-1	M24067	5′-CCCTTCCAGAGTCCCATACA-3′	5′-CAGGCGTGTCAGCTCATTTA-3′	485	60
uPA	NM013085	5′-CAGATCCGATGCTCTTAGCC-3′	5′-GCTGCTCCACCTCAAACTTC-3′	458	61
β-actin	NM031144	5′-AGCCATGTACGTAGCCATCC-3′	5′-CTCTCAGCTGTGGTGGTGAA-3′	228	60

Temp, temperature.