Phytomedicinal Role of Pithecellobium dulce against CCl₄-mediated Hepatic Oxidative Impairments and Necrotic Cell Death

Figure 8

Representative flow cytometric analysis of hepatocytes from experimental animals. Vehicle: hepatocytes from normal animals, AEPD: hepatocytes from the animals treated with AEPD only; CCl₄: hepatocytes from the CCl₄ intoxicated animals; AEPD + CCl₄: hepatocytes from the animals treated with AEPD prior to CCl₄ intoxication; CCl₄ + AEPD: hepatocytes from the animals treated with AEPD post-CCl₄ intoxication, Recovery: hepatocytes from the animals of recovery group and NaAsO₂: hepatocytes from the NaAsO₂ intoxicated (100 ppm in drinking water, 10 weeks) animals (Positive control). Dual parameter dot plot of FITC-lebelled Annexin V fluorescence (x-axis) versus PI-fluorescence (y-axis) has been shown in logarithmic fluorescence intensity. Quadrants: lower left, live cells; lower right, apoptotic cells; upper left, necrotic cells. Data are representative of three independent experiments.

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