Influence of Oreocnide integrifolia (Gaud.) Miq on IRS-1, Akt and Glut-4 in Fat-Fed C57BL/6J Type 2 Diabetes Mouse Model
Figure 7
Guinea pig anti-insulin antibody (Linco Research Inc, MO) and mouse antiglucagon (Sigma) antibodies were used at 1 : 100 dilutions. Alexa Flour-488 and Alexa Flour 546 F(ab)2 secondary antibodies (Molecular Probes) were used at 1 : 200 dilution. Hoecsht 33342 was used to visualize nuclei. Confocal images were captured using a Zeiss LSM 510 laser scanning microscope using a ×63/1.3 oil objective with optical slices ~0.8 μm.
