Keishibukuryogan Reduces Renal Injury in the Early Stage of Renal Failure in the Remnant Kidney Model

Table 1

Summary of primer sequences, PCR conditions and size of amplified fragments.

Genes	Orientation	Sequences (5′ to 3′)	Annealing	Size
MCP-1	Sense	GCC AGA TCT CTC TTC CTC CA	62°C, 60 s	432 bp
	Antisense	GAG GTG GTT GTG GAA AAG AG
VCAM-1	Sense	CTG ACC TGC TCA AGT GAT GG	60°C, 50 s	260 bp
	Antisense	GTG TCT CCC TCT TTG ACG CT
Osteopontin	Sense	AGA GGA GAA GGC GCA TTA CA	61°C, 60 s	498 bp
	Antisense	GCA ACT GGG ATG ACC TTG AT
TGF-𝛽1	Sense	TAC AGG GCT TTC GCT TCA GT	61°C, 60 s	394 bp
	Antisense	TGG TTG TAG AGG GCA AGG AC
Fibronectin	Sense	TTA TGA CGA CGG GAA GAC CTA	56°C, 60 s	295 bp
	Antisense	GGC TGG ATG GAA AGA TTA CTC
GAPDH	Sense	GTG AGG TGA CCG CAT CTT CT	56°C, 60 s	278 bp
	Antisense	TGG AAG ATG GTG ATG GGT TT

MCP-1, monocyte chemoattractant protein-1; VCAM-1, vascular cell adhesion molecule-1; TGF-𝛽₁, transforming growth factor-𝛽₁; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.