San-Huang-Xie-Xin-Tang Prevents Rat Hearts from Ischemia/Reperfusion-Induced Apoptosis through eNOS and MAPK Pathways
Figure 2
SHXT attenuated ischemia/reperfusion (I/R)-induced myocardial apoptosis by the DNA ladder and Bcl-2/Bax ratio. (a) DNA fragmentation was detected by agarose gel electrophoresis. M represents marker; lane 1 represents sham tissue; lane 2 represents vehicle-treated tissue; lane 3 represents SHXT 10 mg/kg-treated tissue (i.v.); lane 4 represents SHXT 30 mg/kg-treated tissue (i.v.); lane 5 represents SHXT 10 mg/kg-treated tissue (oral); lane 6 represents SHXT 30 mg/kg-treated tissue (oral). Similar results were obtained in four other experiments. (b) Quantitative analysis on the Bcl-2/Bax ratio by western blots. Each value represents the mean ± SEM of six rats. versus sham group; , ‡ versus vehicle-treated rats. ANOVA followed by Dunnett’s test.
