The Budding Yeast “Saccharomyces cerevisiae” as a Drug Discovery Tool to Identify Plant-Derived Natural Products with Anti-Proliferative Properties
Figure 3
Phenotypic analysis. (a) Representative images of asynchronously growing wild-type cells in the absence or presence of Lyc for 8 h. Cells treated with Lyc exhibit strong morphological alteration and fragmented nuclei. For each field of cells, differential interference contrast (left) and DAPI staining of DNA (right) images were taken. Specific cell morphologies at different stages of the cell cycle are shown for the no drug control. (b) FACS profile of cells from the experiment in (a). (c) Budding index was measured for growing wild-type cells from the experiment in (a). Cells were counted in each of two experiments, and the average of the two experiments is showed.
