Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of Antrodia camphorate
Figure 4
Time- and dose-dependent SY-1-induced G0/G1 phase arrest and apoptosis in COLO 205 cells. (a) COLO 205 cells were synchronized with 0.04% FCS for 24 h as described in “Materials and Methods” section. After synchronization, the cells were released into complete medium (10% FCS) containing 0.05% DMSO (left panel), or 150 μM SY-1 in 0.05% DMSO (right panel). The percentages of cells in the Sub-G1, G0/G1, S and G2/M phases of the cell cycle were determined using the well-known CellFIT DNA analysis software. Three samples were analyzed in each group, and values represent the mean ± SE (b). Dose-dependent effects of SY-1 on cell cycle and apoptosis in human cancer cells. FACS analysis of DNA content 15 h after release from quiescence by incubation in culture media supplemented with 10% FCS and various concentrations of SY-1 in 0.05% DMSO. The percentage of cells in the Sub-G1, G0/G1, S and G2/M phases of the cell cycle were determined using the CellFIT DNA analysis software. Three samples were analyzed in each group, and values represent the mean ± SE.
