Antiproliferative and Proapoptotic Effects of Labisia pumila Ethanol Extract and Its Active Fraction in Human Melanoma HM3KO Cells
Figure 3
Representative images from morphological observation of SF2Lp-treated and -untreated HM3KO cells stained with acridine orange-ethidium bromide (AO-EB). HM3KO cells were treated with 7.59 μg/mL SF2Lp for 24 (b), 48 (c), and 72 (d) hours. DMSO-treated HM3KO cells served as negative control (a). SF2Lp-treated cells showed significant morphological changes including nuclear condensation (blue arrows), membrane blebbing (yellow arrows), and apoptotic bodies (green arrows). Magnification for (a): 400x; (b)–(d): 1000x.
