Synergistic Cytotoxic Effects of Ganoderma lucidum and Bacillus Calmette Guérin on Premalignant Urothelial HUC-PC Cells and Its Regulation on Proinflammatory Cytokine Secretion
Figure 4
Dose-response curves of (a) BCG and (b) GLe generated by nonlinear regression, in order to define the sinGLe-agent effects as . On day 6 following drug cessation, and since only selected BCG concentrations were tested, the BCG cytotoxicity showed ranged from 60–73% when compared with the solvent control (i.e., BCG 0 CFU on day 6). In order to minimize the error, was used for isobologram analysis. Isobolograms (c) were plotted for treatments 3 and 4 with the determined for BCG and GLe. Concentrations of GLe and BCG were reflected on x- and y-axes, respectively. Area below the additive isobole (the line joining and ) indicates synergistic interaction. Combination indices (CI) were calculated as according to Zhao et al. [14]. Specifically, and are the concentrations of BCG and GLe used in treatments 3 and 4 to achieve 70% drug effect. (fixed as CFU for all experiments) and (19.12 μg/mL in treatment 3; 12.37 μg/mL in treatment 4) are the concentrations for sinGLe agents to achieve the same effect.
