The Inhibition of Spinal Astrocytic JAK2-STAT3 Pathway Activation Correlates with the Analgesic Effects of Triptolide in the Rat Neuropathic Pain Model
Figure 4
Western blot quantification of the expression of GFAP, pJAK2, and pSTAT3 after intrathecal administration of triptolide. (a) SNL injury resulted in increased GFAP expression at POD 6 in the ipsilateral SDH. SNL-induced elevations in GFAP expression were completely inhibited in rats intrathecally injected with triptolide (10 μg/kg). (b) The levels of the phosphorylated forms of JAK2 (pJAK2) and STAT3 (pSTAT3) were increased in the ipsilateral SDH of SNL rats at POD 6. The intrathecal administration of triptolide (10 μg/kg) prevented SNL-induced pJAK2 and pSTAT3 accumulation. SNL, spinal nerve ligation; SDH, spinal dorsal horn; POD, postoperative day. *, SNL-Veh group versus Sham-Veh group; #, SNL-T10 group versus SNL-Veh group. The results are expressed as relative fold changes compared to the Sham-Veh group. Each group consisted of 12 rats.
