Molecular Mechanism of Isocupressic Acid Supresses MA-10 Cell Steroidogenesis

Table 1

Primer pairs for semiquantitative real-time PCR.

Target gene	GenBank accession number	Primer sequence	Product size (bp)
StAR	NM_011485.4	CGTGAGCGTGCGCTGTACCA	95
		TGACACCACTCTGCTCCGGCA
P450scc	NM_019779.3	ACCGAGATGCTGGCAGGAGGG	119
		CGGGCAGCCAGGACTTCAGC
3β-HSD	NM_008293.3	CAGCCAGGGGCCTTCGAGAC	143
		GCTGGCATTAGGGCGGAGCC
PBR	NM_009775.4	GCTGGCTTTTGCCACCGTGC	119
		TGGCTGGCAGGGCTGCATTC
β-actin	NM_007393.3	CCACCCGCGAGCACAGCTTC	102
		CGTTGTCGACGACCAGCGCA

3β-HSD: 3β-hydroxysteroid dehydrogenase isoform: P450scc, cytochrome P450 cholesterol side-chain cleavage: PBR, peripheral-type benzodiazepine receptor: StAR, steroidogenic acute regulatory protein.