Lignosus rhinocerus (Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth in PC-12 Cells

Figure 2

Percentage of neurite-bearing cells in the cell line PC-12 incubated with various concentrations of L. rhinocerus aqueous extract (μg/mL). Neurite growth (vertical axis) was quantified as the percentage of cells bearing axodendritic processes longer than two times of cell diameters in length. Negative control consists of PC-12 cells in medium only whereas in positive control the cells were treated with 50 ng/mL (w/w) NGF. Data are expressed as means ± standard deviation (𝑛=3). Means with different alphabets show significant difference (𝑃<0.01; Duncan’s Multiple Range Test (DMRT)) (permuted-𝑃<0.001).