Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways
Figure 1
(a) Viability of CL1-5 cells incubated with EEAC (0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16, 32, 64, and 128 μg/mL) for 24 h and 36 h. Cell viability was measured using MTT assay and is expressed as % of cell survival relative to control. (b) Flow cytometric analysis of EEAC on the cell cycle of CL1-5. All the cells were treated with 1% fetal bovine serum with the addition of EEAC at 0.125 μg/mL, 0.25 μg/mL, 0.5 μg/mL, and 1.0 μg/mL for 36 h. The value on the -axis represents the DNA content, while the shaded area indicates the percentage of cells at the S phase. Percentage of sub G1 contents in CL1-5 cells treated with EEAC. Values are means of three separate experiments, with standard deviation represented by vertical bars. ; . Lower case for 24 h and upper case for 36 h.
