Ethanol Extracts of Fruiting Bodies of <i>Antrodia cinnamomea</i> Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways

<table>Effects of EEAC on protein levels of MAPK signaling and phosphorylated focal adhesion kinase (p-FAK) in CL1-5 cells. (a) CL1-5 were treated with several different concentrations of EEAC (0.125, 0.25, 0.5, and 1.0 <i>μ</i>g/mL) for 36 h. (b) Effects of PI3K inhibitor (LY294002; 50 <i>μ</i>M), ERK1/2 inhibitor (PD98059; 50 <i>μ</i>M), JNK inhibitor (SP600125; 50 <i>μ</i>M), and p38 MAPK inhibitor (SB203580; 50 <i>μ</i>M) on EEAC-induced protein expressions of MMP-2 and -9. Cells were pretreated with an inhibitor (50 <i>μ</i>M) at 1 h prior to the treatment with 1.0 <i>μ</i>g/mL EEAC for 36 h (total inhibitor exposure time was 37 h). Protein expressions of MMP-2 and -9 were determined by the western blotting assay.</table>

Evidence-Based Complementary and Alternative Medicine

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Figure 7

Figure 7: Ethanol Extracts of Fruiting Bodies of <i>Antrodia cinnamomea</i> Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways 

Figure 7 | Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways 