Evidence-Based Complementary and Alternative Medicine

Research Article

Anti-Inflammatory Activities of a Chinese Herbal Formula IBS-20 In Vitro and In Vivo

Table 2

Primer sequences for real-time quantitative PCR.
GenePrimer sequences (5′ to 3′)
IFNγForward, TGGCTGTTTCTGGCTGTTACTG
Reverse, AGGTGTGATTCAATGACGCTTATG
IL-1βForward, TCTATACCTGTCCTGTGTAATG
Reverse, GCTTGTGCTCTGCTTGTG
IL-6Forward, TCCATCCAGTTGCCTTCTTG
Reverse, TTTCTCATTTCCACGATTTCCC
IL-10Forward, TCTCCCCTGTGAAAATAAGAG
Reverse, GCCTTGTAGACACCTTGG
IL12p35Forward, TTGATGATGACCCTGTGCCTTGG
Reverse, GATTCTGAAGTGCTGCGTTGATGG
IL-12p40Forward, TGAGAACTACAGCACCAGCTTCTT
Reverse, CTTCAAAGGCTTCATCTGCAAGT
IL-23p19Forward, CAACTTCACACCTCCCTAC
Reverse, CCACTGCTGACTAGAACTC
NOS-2Forward, CGGAGCCTTTAGACCTCAACA
Reverse, CCCTCGAAGGTGAGCTGAAC
TGF-β Forward, CTAATGGTGGACCGCAACAAC
Reverse, GCACGGGACAGCAATGGG
TLR4Forward, ACCAAGAACATAGATCTGAGCTTCAA
Reverse, ATGCCATGCCTTGTCTTCAATT
TNFαCCTCCCTCTCATCAGTTCTATGG
Reverse, GGCTACAGGCTTGTCACTCG