Inhibitory Effects of Chung Hun Wha Dam Tang (CHWDT) on High-Fat Diet-Induced Obesity via AMP-Activated Protein Kinase Activation
Figure 3
CHWDT induces lipolytic gene CPT1, ACO and LCAD expression in hepatocytes. AML cells were pretreated with CHWDT (50 and 100 g/mL) for 1 h and palmitate (0.45 mM) was used for 12 h and then CPT1, ACO and LCAD mRNA levels were observed by RT-PCR (a) HepG2 cells were pretreated with CHWDT (50 and 100 g/mL) for 1 h and palmitate (0.45 mM) was used for 12 h and then CPT1 mRNA levels were performed by RT-PCR (b) CPT1, ACO and LCAD mRNA levels were analyzed from liver tissue collected high fat diet induced obese mice (c) The representative bands are shown from at least three independent experiments.
