Inhibitory Effects of Chung Hun Wha Dam Tang (CHWDT) on High-Fat Diet-Induced Obesity via AMP-Activated Protein Kinase Activation
Figure 4
CHWDT decreases gluconeogenic gene PGC1α, G6Pase, and PEPCK expression in hepatocytes. PGC1, G6Pase, and PEPCK mRNA levels in HepG2 cells (a) were observed. And also, representative immunoblot for PGC1 protein expression was shown (b) In AML cells, palmitate-induced PGC1 mRNA levels (c) were decreased by CHWDT treatment. After the pretreatment of 50 and 100 g/mL CHWDT for 1 h, palmitate was treated with a concentration of 0.45 mM for 24 h. PGC1α, G6Pase and PEPCK mRNA levels were analyzed from liver tissue collected from high fat diet-induced obese mice (d) Analysis of mRNA was done by RT-PCR. The representative bands are shown from at least three independent experiments.
