Inhibitory Effects of Chung Hun Wha Dam Tang (CHWDT) on High-Fat Diet-Induced Obesity via AMP-Activated Protein Kinase Activation
Figure 5
CHWDT suppresses iNOS, TNF-α, and NO levels. TNF- and iNOS mRNA levels (a) and iNOS protein expression (b) in Raw264.7 cells and TNF- and iNOS mRNA levels in HepG2 cells (c), where CHWDT (50 and 100 g/mL) was pretreated for 1 h and LPS (1 g/mL) was used for 24 h, mRNA was checked by RT-PCR and protein expression by western blotting. Data are representative of at least three independent experiments. NO production in Raw264.7 cells was observed by using Griess reagents, where CHWDT (50 and 100 g/mL) was pretreated for 1 h and LPS (1 g/mL) was used for 24 h (d) Data are presented as mean ± SEM of three independent experiments with triplicate wells and as ***.
