7-Hydroxy--Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation
Figure 6
Observation on the effects of CT1 treatment over 24 h on MCF-7 protein levels using Western blot. (a) CT1 was found to decrease ERK1/2 and anti-apoptotic Bcl-2 and Bim protein levels, while increasing FasL protein levels. XIAP and proapoptotic Bax protein levels were unaffected following CT1 exposure. β-actin was used as a normalization control for all experiments. (b) Quantification of protein band intensities were determined by densitometry analysis and normalized to β-actin using the ImageJ v1.43 software. All results were presented as mean normalized intensity ± SEM of three independent experiments.
