Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells
Figure 4
Effects of EBNE on MAPKs. (a) hADSCs were starved for 24 h and treated with 2,000 ppm EBNE for the indicated times. Total cell lysate was then prepared at 0, 5, 15, and 30 min following EBNE stimulation and subjected to Western blot analysis. The bands for phospho-p44/42, phospho-p38 MAPK, phospho-SAPK/JNK, and phosphor-IκB-α were detected and normalized to their total form and β-actin. Results are shown as mean ± SD. * versus untreated controls (0 time point). Data are representative of at least three independent experiments. (b, c) hADSCs were starved for 24 h and then treated with 50 μM PD98059 or 5 μM SB203580 or 20 μM PDTC in the presence of EBNE. The cell lysate was prepared at 5 min (upon treatment of PD98059, PDTC) and 30 min (upon treatment of SB203580) and then subjected to Western blot analysis. The bands for phospho-p44/42, phospho-p38 MAPK, and phosphor-IκB-α were detected and normalized to their total form and β-actin. Results are shown as mean ± SD. # versus untreated controls. ** versus EBNE (2,000 ppm) only. Data are representative of at least three independent experiments.
