RETRACTED: Potential Therapeutic Role of Z-Isochaihulactone in Lung Cancer through Induction of Apoptosis via Notch Signaling
Figure 4
Z-isochaihulactone induced in vivo growth inhibition of A549 xenografts in nude mice. Inhibition of tumor growth from A549 cells for upregulat of Notch1 protein. Nude mice injected with approximately 1 × 107 A549 cells into the dorsal subcutaneous tissue. (a) When the tumor volumes reached 80–120 mm3, A549 tumor-bearing mice were administered sc. with vehicle control (●), 15 mg/kg Z-isochaihulactone (■), and 30 mg/kg Z-isochaihulactone (▲) on days 1–5 for 5 days. These figures show A549 cells relative tumor volume of control and therapeutic groups. (b) Immunohistochemical staining was analyzed in A549 tumor tissues (at day 6 after initiation of drug treatment). Representative photographs of sections of the control group and Z-K8-treated group. A549 tumors, immunohistochemically stained with NICD and Cleaved caspase-3 rabbit monoclonal antibody, and NICD and Cleaved caspase-3 positive cells were stained brown (black arrowheads) (×400). [Scale bars, 100 μm. different experiments.] Means ± SD. bP < 0.05, cP < 0.01 versus the control. (d) Western blot was analyzed in A549 tumor tissues (at day 34 after initiation of drug treatment). Tumor lysate was probed with Cleaved caspase-9, caspase-9, Cleaved caspase-3, caspase-3, Cleaved PARP, PARP, and NICD antibodies. Expression of β-actin was used as an internal control.
