Sphingosine Kinase-1-Dependent and -Independent Inhibitory Effects of Zanthoxyli Fructus to Attenuate the Activation of Mucosal Mast Cells and Ameliorate Food Allergies in Mice
Figure 1
Inhibitory effect of the water extracts of Arecae Semen, Cinnamoni Cortex, Curcumae Rhizoma, Rhei Rhizoma, and Zanthoxyli Fructus on degranulation that was induced by antigens in the RBL-2H3 cells. (a) The time scheme for the degranulation assay. (b) RBL-2H3 cells that were sensitized with 0.5 μg/mL anti-DNP IgE (24 h) were incubated with herbal medicine extracts for 30 min. The cells were stimulated with (filled) or without (open) 100 ng/mL DNP-BSA for 1 h, and β-hexosaminidase release was determined. (c) The inhibitory effect of antiallergic drugs (10 μM) on DNP-BSA-induced degranulation was examined. The data are expressed as the mean ± SD (; B, C). * compared with the vehicle (b, c). (d) The effect of ZF (0.32 mg/mL) treatment on the increase of [Ca2+]i that was induced DNP-BSA. The RBL-2H3 cells that were sensitized with IgE were labeled with Fura-2 AM and treated with ZF or vehicle for 30 min. The Ca2+-mobilization was determined after stimulation with DNP-BSA using F4500. The data are representative of at least three independent experiments.
