Sphingosine Kinase-1-Dependent and -Independent Inhibitory Effects of Zanthoxyli Fructus to Attenuate the Activation of Mucosal Mast Cells and Ameliorate Food Allergies in Mice
Figure 5
The effect of ZF (0.32 mg/mL) treatment on A23187-induced [Ca2+]i elevation and degranulation. (a) The time scheme for the degranulation assay. (b) RBL-2H3 cells were labeled with Fluo-3 AM and incubated with ZF or vehicle for 30 min. The cells were stimulated with 25 μM A23187 and monitored by calcium imaging. The data are representative of at least three independent experiments. (c) The RBL-2H3 cells were incubated with ZF or vehicle for 30 min, the cells were stimulated with (filled) or without (open) A23187 for 30 min, and β-hexosaminidase release was determined. (d) The mBMMCs were incubated with ZF (0.32 mg/mL), DMS (10 μM), cyclosporin A (1 μM), W-7 (32 μM), KN-93 (32 μM), or vehicle for 30 min, and the cells were stimulated with 25 μM A23187 for 30 min. β-hexosaminidase release was determined. The data are expressed as the mean ± SD (; c, d). * compared with the vehicle (c, d).
