Sphingosine Kinase-1-Dependent and -Independent Inhibitory Effects of Zanthoxyli Fructus to Attenuate the Activation of Mucosal Mast Cells and Ameliorate Food Allergies in Mice
Figure 6
The effect of ZF and DMS on the development of FA in vivo. (a) The induction of allergic diarrhea was compared between the vehicle- and ZF-treated groups. ZF was orally administered during the induction of allergic diarrhea by OVA oral challenge as described in the Materials and Methods section (). (b) DMS (1 mg/kg) suppressed the incidence of allergic diarrhea ( 5–8). (c) The proximal colons of the vehicle, ZF (320 mg/kg), and DMS (1 mg/kg) treated mice after oral challenge with OVA were stained with anti-mMCP1 antibody. The scale bar represents 200 μm. (d) The expression levels of IL-4 and sphk1 in the proximal colons were measured using real-time PCR. The results are expressed as the relative ratio to the proximal colons of the vehicle-treated FA mice. The data are expressed as the mean ± SD (). * compared with the vehicle.
