Evidence-Based Complementary and Alternative Medicine

Research Article

Sphingosine Kinase-1-Dependent and -Independent Inhibitory Effects of Zanthoxyli Fructus to Attenuate the Activation of Mucosal Mast Cells and Ameliorate Food Allergies in Mice

Table 2

List of genes selected by microarray analysis. The fold-change log₂ ratio was calculated as the log₂ ratio of the expression level in the activated mBMMCs compared to the expression in the normal mBMMCs or as the log₂ ratio of the expression level in the ZF mBMMCs compared to that in the activated mBMMCs. (a) Upregulation (>2 fold) of genes by DNP-BSA stimulation and downregulation (>2 fold) after ZF pretreatment. (b) Downregulation (>2 fold) of genes by DNP-BSA stimulation and upregulation (>2 fold) after ZF pretreatment.


Gene name	Gene description	Fold-change log₂ ratio
Gene name	Gene description	IgE + DNP/Normal	ZF/IgE + DNP

(a)
Tnip3	TNFAIP3 interacting protein 3	4.47	−3.36
Sphk1	Sphingosine kinase 1	2.50	−2.65
Chac1	ChaC, cation transport regulator-like 1 (E. coli)	1.80	−2.40
Trib1	Tribbles homolog 1 (Drosophila)	3.21	−2.39
Uhrf1bp1l	UHRF1 (ICBP90) binding protein 1-like	1.82	−2.23
Car2	Carbonic anhydrase 2	2.16	−1.86
Socs3	Suppressor of cytokine signaling 3	1.89	−1.82
Sema7a	Sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A	2.25	−1.77
Fermt2	Fermitin family homolog 2 (Drosophila)	2.81	−1.75
Zfp57	Zinc finger protein 57	1.90	−1.71
Klf9∣Gm9971	Kruppel-like factor 9 ∣ predicted gene 9971	1.42	−1.68
Mustn1	Musculoskeletal, embryonic nuclear protein 1	1.57	−1.64
Tnfsf8	Tumor necrosis factor (ligand) superfamily, member 8	2.48	−1.64
Gimap5	GTPase, IMAP family member 5	1.41	−1.61
Dusp18	Dual-specificity phosphatase 18	1.08	−1.58
Slco4a1	Solute carrier organic anion transporter family, member 4a1	2.25	−1.57
Fhl2	Four and a half LIM domains 2	1.06	−1.56
Spry2	Sprouty homolog 2 (Drosophila)	2.64	−1.53
A630033H20Rik	RIKEN cDNA A630033H20 gene	2.34	−1.52
Zc3h12a	Zinc finger CCCH type containing 12A	1.70	−1.51
Nfkbia	Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha	1.92	−1.50
Il2	Interleukin 2	1.91	−1.50
Zc3h12c	Zinc finger CCCH type containing 12C	2.28	−1.47
Marcksl1	MARCKS-like 1	2.88	−1.42
Hbegf	Heparin-binding EGF-like growth factor	4.90	−1.41
Csf2	Colony stimulating factor 2 (granulocyte macrophage)	5.30	−1.41
Erf	Ets2 repressor factor	2.10	−1.40
Kcnk5	Potassium channel, subfamily K, member 5	1.13	−1.38
Mmd	Monocyte to macrophage differentiation associated	2.05	−1.37
Phlda1	Pleckstrin homology-like domain, family A, member 1	3.10	−1.32
Zc3h12c	Zinc finger CCCH type containing 12C	2.26	−1.31
Rilpl2	Rab interacting lysosomal protein-like 2	1.48	−1.31
Faah	Fatty acid amide hydrolase	2.35	−1.29
Fst∣Thrap3	Follistatin ∣ thyroid-hormone-receptor-associated protein 3	1.67	−1.19
Insig1	Insulin-induced gene 1	1.65	−1.19
Spry1	Sprouty homolog 1 (Drosophila)	4.36	−1.19
Pde12	Phosphodiesterase 12	1.29	−1.19
Rc3h1	RING CCCH (C3H) domains 1	1.13	−1.18
Dusp2	Dual-specificity phosphatase 2	2.15	−1.15
F3	Coagulation factor III	1.16	−1.13
Eda2r	Ectodysplasin A2 receptor	1.65	−1.11
Prrg4	Proline-rich Gla (G-carboxyglutamic acid) 4 (transmembrane)	1.95	−1.11
Traf6	TNF receptor-associated factor 6	1.03	−1.08
Peli1	Pellino 1	1.48	−1.07
Cxcl2	Chemokine (C-X-C motif) ligand 2	3.91	−1.04
Ptger4	Prostaglandin E receptor 4 (subtype EP4)	1.06	−1.03
Siah2	Seven in absentia 2	1.20	−1.02
Chka	Choline kinase alpha	1.58	−1.01
Ehd4	EH-domain containing 4	1.10	−1.01

(b)
Cbfa2t3	Core-binding factor, runt domain, alpha subunit 2, translocated to 3 (human)	−1.59	1.71
ATP6∣Gm10925	ATP synthase F0 subunit 6 ∣ predicted gene 10925	−1.20	2.01
Nynrin	NYN domain and retroviral integrase containing	−1.11	1.00
Hist1h1c	Histone cluster 1, H1c	−1.01	1.15
Arrdc3	Arrestin domain containing 3	−1.10	1.36
Gfi1b	Growth-factor-independent 1B	−1.52	1.40