HRT, Herbal Formula, Induces G2/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells
Figure 4
Induction of apoptosis by HRT in HCT116 cells. (a) The effect of HRT on the expression of pro-apoptotic proteins in HCT116 cells. After treatment with HRT (50, 100, or 300 μg/mL) for 24 h, cell lysates were prepared for Western blot analysis against caspase-3, -8, -9, Bid, Bcl-2, PARP, and β-actin. (b) The DNA fragmentation was observed in HCT116 cells treated with various concentrations of HRT. DNA fragmentation with a ladder pattern is a characteristic of apoptosis.
