Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG

Figure 3

Natural product screening to reduce NANOG⁺ subpopulation of MCF-7 cells. ((a) and (b)) Screening for natural products by reducing NANOG⁺ population assay. MCF-7 cells and MDA-MB231 cells (9×10⁴ cells in 12-well plates) were treated with natural products for 72 hours before NANOG levels were measured. Total NANOG⁺ cells were calculated and data were shown as mean ± SEM from 3 independent experiments. ***𝑃<0.001 or **𝑃<0.01 versus control cells. (c) Immunofluorescent staining of NANOG (green) and MUC1 (red) on control, treated with ugonins (J, K), NANOG-overexpressing, and NANOG-knockdown MCF-7 cells.

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