Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
Figure 4
TET inhibits in vivo tumor metastasis in mouse breast cancer model. (a) Bioluminescence imaging of TET treated tumor bearing mice. Firefly luciferase-tagged 4T1 cells were injected into the MFP of BALB/c mice and TET was orally administered (10 mg/kg body weight, once every two days); images were obtained by using IVIS 200 imaging system. Representative Xenogen imaging results at week 4 are shown. (b) TET reduced tumor lung metastasis sites in 4T1 tumor-bearing mice. Picture shows the number of lung metastasis sites on the lung surface for each mouse. The black line shows the average number of metastasis sites for each group. (c) Photograph of pulmonary metastases. Animals were sacrificed after 4 weeks, and the lungs were dissected and photographed. (d) Histological examination of lung samples. Lung tissues sections were stained with H&E stain, and photographs were made under a microscope at a magnification of 400x (T: tumor).
