Research Article
Effect of Berberine on PPARα/NO Activation in High Glucose- and Insulin-Induced Cardiomyocyte Hypertrophy
Table 2
Effect of berberine on cardiomyocyte hypertrophy induced by high glucose and insulin (HGI).
| | Group (μmol/L) | Cell surface area | Protein level | ANF mRNA | | (μm2/cell; n = 3) | (μg/106 cell; n = 6) | (n = 3) |
| | Control | 459.7 ± 64.1 | 23.1 ± 5.3 | 30.6 ± 6.4 | | HGI | 1229.6 ± 99.5* | 47.3 ± 3.6* | 179.4 ± 25.5* | | HGI + berberine (3) | 711.2 ± 43.0# | 37.4 ± 2.0# | 81.8 ± 7.5# | | HGI + fenofibrate (0.3) | 678.9 ± 21.3# | 33.8 ± 6.0# | 37.9 ± 9.5# | | HGI + MK886 (0.3) | 1208.8 ± 27.1 | 49.9 ± 5.9 | 184.7 ± 22.3 | | HGI + fenofibrate (0.3) + MK886 (0.3) | 1212.4 ± 82.7● | 50.2 ± 5.1● | 161.5 ± 36.5● | | HGI + berberine (3) + MK886 (0.3) | 1257.4 ± 29.6▲ | 47.2 ± 3.3▲ | 154.0 ± 17.6▲ | | HGI + L-arginine (100) | 768.1 ± 49.0# | 38.6 ± 4.1# | 80.7 ± 8.7# | | HGI + L-NAME (100) | 1213.1 ± 84.0 | 48.1 ± 3.5 | 147.3 ± 20.4 | | HGI + L-arginine (100) + L-NAME (100) | 1199.9 ± 75.7∆ | 48.8 ± 4.9∆ | 163.3 ± 20.8∆ | | HGI + berberine (3) + L-NAME (100) | 972.4 ± 41.1▲ | 43.9 ± 2.1▲ | 110.0 ± 9.5▲ |
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ANF: atrial natriuretic factor; L-NAME: -nitro-L-arginine-methyl ester. Results are mean ± SEM of n independent experiments. *P < 0.05 versus control; #P < 0.05 versus HGI; ●P < 0.05 versus HGI + fenofibrate (0.3 μmol/L); ▲P < 0.05 versus HGI + berberine (3 μmol/L); ∆P < 0.05 versus HGI + L-arginine (100 μmol/L).
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