Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation

Figure 1

HRT inhibits adipocyte differentiation of 3T3-L1 cells. Two-day postconfluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of HRT extract and were replete every 2 days along with relevant media cocktail up to day 8. Cells treated with 1X PBS were used as control. (a) Intracellular lipids were stained with Oil red O. (b) Cell viability was determined by MTT assay. (c) Inhibition of signaling (Erk and Akt) inhibitors (PD98059 and LY294002) in accumulation of intracellular lipids. The results were confirmed by three independent experiments, which were each conducted in triplicate. Data are expressed as the mean ± S.D. **𝑃<0.01 versus controls (cell viability), ***𝑃<0.001 versus controls (cell viability), ^$𝑃<0.05 versus control, and ^$$$𝑃<0.001 versus control (Oil red O). Bar indicates 100 μm.

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