Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation

<table>Inhibitory effect of major constituents of HRT in adipocyte differentiation of 3T3-L1 cells. 3T3-L1 preadipocytes were treated with or without major constituents of HRT (0–500 <i>μ</i>g/mL) during differentiation for 8 days. Oil red O staining of 3T3-L1 cells treated with (a) berberine, (b) palmatine, (c) geniposide, (d) wogonin, (e) baicalein, and (f) baicalin was determined. Each value was expressed as a percentage of the differentiated control (100%). The data show the mean ± S.D. of three experiments. Oil red O: <svg height="13.95" id="M34" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 73.300003 13.95" width="73.300003" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus control; <svg height="13.95" id="M35" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 88.449997 13.95" width="88.449997" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus control. </table>

Evidence-Based Complementary and Alternative Medicine

fig7

Figure 7

Figure 7: Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation 