<i>In Vitro</i> Screening for Antihepatic Steatosis Active Components within Coptidis Rhizoma Alkaloids Extract Using Liver Cell Extraction with HPLC Analysis and a Free Fatty Acid-Induced Hepatic Steatosis HepG2 Cell Assay

<table>The HPLC chromatograms of HepG2 cell extracts analyzed at 270 nm following treatment with (a) CAE, (b) a mixed standard of seven alkaloids, or (c) gemfibrozil. The lines for ((A)–(D)) represent the (A) sample work solution, (B) the denatured desorption elute of HepG2 cells incubated with sample, (C) the final washing elute of HepG2 cells incubated with sample, and (D) the denatured desorption eluate of HepG2 cells cultured in medium without sample (blank). Peaks identified as (<b>1</b>) magnoflorine, (<b>2</b>) columbamine, (<b>3</b>) epiberberine, (<b>4</b>) jateorhizine, (<b>5</b>) coptisine, (<b>6</b>) palmatine, and (<b>7</b>) berberine.</table>

Evidence-Based Complementary and Alternative Medicine

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Figure 2: <i>In Vitro</i> Screening for Antihepatic Steatosis Active Components within Coptidis Rhizoma Alkaloids Extract Using Liver Cell Extraction with HPLC Analysis and a Free Fatty Acid-Induced Hepatic Steatosis HepG2 Cell Assay 

Figure 2 | In Vitro Screening for Antihepatic Steatosis Active Components within Coptidis Rhizoma Alkaloids Extract Using Liver Cell Extraction with HPLC Analysis and a Free Fatty Acid-Induced Hepatic Steatosis HepG2 Cell Assay 