Microarray Analysis Reveals the Molecular Basis of Antiarthritic Activity of Huo-Luo-Xiao-Ling Dan
Figure 1
HLXL alters Bhsp65-induced gene expression in LNC. The draining lymph nodes were harvested from HLXL-treated (TH) and vehicle (Water-) treated (TW) arthritic LEW rats after 7 days of treatment. The lymph node cells (LNC) were cultured in vitro with or without Bhsp65. Thereafter, total RNA was extracted from cells and then used for further generation of cRNA for testing in microarrays. The gene expression profiles of Bhsp65-restimulated LNC are represented by heat map (a) and Venn diagram (b). LNC cultured in medium alone (ex vivo) served as the baseline for the Bhsp65-restimulated group. In heat map, red color indicates increased expression, whereas blue color indicates reduced expression of genes compared with the level of the corresponding baseline gene expression. DEG were selected using as cut-off fold change >2.0 and value corrected with Benjamin-Hochberg (Med: medium; Bhsp65: mycobacterial heat shock protein 65).
