Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells
Figure 3
Regulation of Mcl-1 and PUMA by destruxin B. (a) Regulation of Bcl-2 family proteins by destruxin. A549 cells were treated with 0, 10, or 20 μM of destruxin B for 12 and 24 h. The cells were collected, lyzed, and subjected to western blot with antibodies against PUMA, Bax, Bak, Mcl-1, Bcl-xL, Bcl-2, and β-actin, respectively. (b) Upregulation of PUMA but not Mcl-1 RNA expression by destruxin. A549 cells were treated with or without 20 μM destruxin B for 8, 12, and 24 h. The cells were then collected and the total RNA was extracted followed by real-time PCR using PUMA (upper panel) or Mcl-1 (bottom panel) primer set. The actin primer set was also applied as an internal control. The normalized relative expression folds of PUMA between untreated and treated cells were quantified ( versus control; versus control; versus control).
