Chlorin e6 Prevents ADP-Induced Platelet Aggregation by Decreasing PI3K-Akt Phosphorylation and Promoting cAMP Production

Figure 2

The inhibitory effect of CE6 on platelet aggregation induced by ADP. Platelets (3×10⁸/mL) were preincubated with or without CE6 (1.63–30 M) in the presence of 1 mM CaCl₂ for 2 min at 37°C. The platelet aggregation was then induced by 10 μM ADP and measured with a turbidimetric aggregometer. The aggregation reaction was terminated after 5 min, and the percent aggregation rate was calculated. Each graph shows the mean ± SEM of four independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 compared to the agonist control.

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