Chlorin e6 Prevents ADP-Induced Platelet Aggregation by Decreasing PI3K-Akt Phosphorylation and Promoting cAMP Production
Figure 8
Effects of CE6 on ADP-induced phosphorylation of MAPKs, PI3K, and Akt and on expression of the PKA catalytic subunit. Washed platelets (/mL) were pre-incubated for 2 min with vehicle or CE6 at the indicated concentration. The platelets were then stimulated with 10 μM ADP for 5 min at 37°C. After terminating the reactions, total cell proteins were extracted. The proteins were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The membranes were then probed with antibodies against phospho-p44/42, p44/42, phospho-p38, p38, phospho-SAPK/JNK, and β-actin (a); and phospho-PI3K, phospho-Akt, Akt, PKAα/β/γ catalytic subunit, and β-actin (b). Antibody binding was visualized by chemiluminescence. All immunoblots are representative of three or four independent experiments.
