Matrine Inhibits Infiltration of the Inflammatory Monocyte Subset in Injured Mouse Liver through Inhibition of Monocyte Chemoattractant Protein-1
Figure 6
Mat inhibited the chemotactic activity of MCP-1. Chemotaxis assay was carried out with transwell culture chambers. Culture mediums containing 10 ng/mL MCP-1 were added to the lower wells of the chambers, and 1 × 105 murine PBMCs were seeded into the upper wells in the absence or presence of the indicated concentrations of Mat. (a) After 2 h incubation, the cells migrating to the lower surface of the membrane were examined under microscope after being fixed with methanol and stained with crystal violet (original magnification ×100). (b) Four different areas of migrated cells were counted for each data point, and the number of migrated cells per well was calculated. Data were obtained from 3 independent experiments and presented as mean ± (SD). versus MCP-1 alone; versus nontreatment control group.
