Eupafolin and Ethyl Acetate Fraction of Kalanchoe gracilis Stem Extract Show Potent Antiviral Activities against Enterovirus 71 and Coxsackievirus A16

Figure 3

Plaque reduction by KGS extract. Monolayer of RD cells on six-well plates infected with EV71 or CVA16 at a titer of 100 pfu was treated simultaneously with indicated KGS extract concentrations. After 1 h incubation, monolayer was covered with 2 mL of agarose overlay medium and incubated for 2 days at 37°C in a CO₂ incubator. Finally, plaques were counted after crystal violet staining.