Magnolol Ameliorates Ligature-Induced Periodontitis in Rats and Osteoclastogenesis: In Vivo and In Vitro Study

Figure 2

Effect of magnolol on the number of TRAP-positive multinucleated cells (MNCs) and the expression of RANKL and OPG in gingival tissues. (a) The micrographs (upper panel: 100x magnification; lower panel: 200x magnification) represent the histological sections around intermolar region of rats 8 days after ligature placement, and the quantitative analysis of the number of TRAP⁺ MNCs with red staining along the bony resorptive lacunae (cells/mm) was performed (scale bar = 50 μm) (c). (b) The protein expression of RANKL and OPG was determined by Western blot in dissected tissues, (d) and the relative protein RANKL/OPG ratio was measured. Data are expressed as mean ± SD (𝑛=5). **𝑃<0.01, ***𝑃<0.001 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus ligation group.

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