7,3′,4′-Trihydroxyisoflavone Ameliorates the Development of Dermatophagoides farinae-Induced Atopic Dermatitis in NC/Nga Mice

Figure 2

Effects of 7,3′,4′-THIF and daidzin on inflammatory cytokine and β-hexosaminidase production. ((a), (b), and (c)) The inhibitory effects of 7,3′,4′-THIF and daidzin on LPS-induced NO, TNF-α, and IL-6 production in RAW 264.7 cells. The cells were treated simultaneously with 2 μg/mL LPS and samples (10, 20, or 40 μM). Cytokine levels were determined by ELISA. (d) The inhibitory effects of 7,3′,4′-THIF and daidzin on anti-DNP IgE-induced β-hexosaminidase production in RBL-2H3 cells. The cells were treated simultaneously with 0.5 μg/mL anti-DNP IgE solution and samples (10, 20, or 40 μM). Data represent means ± SD (𝑛=5). *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 compared with LPS- or anti-DNP IgE-treated cells.

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