Therapeutic Effect of Chung-Pae, an Experimental Herbal Formula, on Acute Lung Inflammation Is Associated with Suppression of NF-κB and Activation of Nrf2
Figure 2
Chung-pae suppresses NF-κB transcriptional activity and NF-κB dependent gene expression. (a) The transcriptional activity NF-κB was measured in an NF-κB reporter cell line derived from RAW 264.7 cells. The cell line was pretreated with indicated amounts of CP for 16 h and then subsequently with TLR4 specific LPS (0.1 μg/mL) for 8 h. Luciferase activity was normalized by the amount of total proteins in cell lysate. Treatment with 5 μg/mL of CP was not statistically significant. * was less than 0.05, compared to the LPS-treated. Data represent the mean ± SEM of three independent experiments. (b) RAW 264.7 cells were treated with CP and LPS as in (a). Total RNA was extracted and analyzed by semiquantitative RT-PCR for TNF-α and IL-1β. The intensity of each PCR band was measured by densitometric analysis (ImageJ), and the relative expression of each gene was calculated over GAPDH. * was less than 0.05, compared to the LPS treated. Data are presented as the mean ± SEM of 3 separate experiments.
