Therapeutic Effect of Chung-Pae, an Experimental Herbal Formula, on Acute Lung Inflammation Is Associated with Suppression of NF-κB and Activation of Nrf2
Figure 3
Chung-pae activates Nrf2 transcriptional activity and induces Nrf2 dependent genes. (a) An Nrf2 reporter cell line, derived from RAW 264.7 cells, was treated with SFN (5 mM) or the indicated amounts of CP for 16 hours. Luciferase activity was normalized by the amount of total proteins. Data are shown in the mean ± SEM of three independent measurements. *, **, and *** were less than 0.05, 0.01, and 0.001, respectively, compared to untreated control. (b) RAW 264.7 cells were treated with CP, similar to (a). Total RNA was analyzed by semiquantitative RT-PCR for NQO-1, HO-1, and GCLC. The intensity of each PCR band was measured by densitometric analysis (ImageJ), and relative expression of each gene was calculated over GAPDH. * and ** were less than 0.05, compared to the untreated. Data are presented as the mean ± SEM of 3 separate experiments.
