Therapeutic Effect of Chung-Pae, an Experimental Herbal Formula, on Acute Lung Inflammation Is Associated with Suppression of NF-κB and Activation of Nrf2
Figure 5
Aerosol intratracheal Chung-pae administration suppresses the expression of Proinflammatory cytokines and enhances the expression of Nrf2-dependent genes in the lungs of mice. Mice (/group) received 5 mg/kg or 20 mg/kg of CP 2 h after an i.p. LPS injection. At 24 h after LPS injection, the lungs of variously treated mice were harvested for semiquantitative RT-PCR analysis of Proinflammatory genes (a). The intensity of each PCR band was measured by densitometric analysis (ImageJ), and relative expression of each gene was calculated over GAPDH. * was less than 0.05, compared to the mice treated with LPS only. (b) Similarly, the expression of Nrf2 dependent genes in the lung was analyzed. Mice, treated with 20 mg/kg of CP, increased the expression of Nrf2 dependent genes. Expressions of these genes were enhanced by CP. * was less than 0.05, compared to untreated control, and ** was less than 0.05, compared to the LPS treated.
