Research Article

The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway

Figure 8

RT-PCR validation of PCR array results. After cells were treated by 0, 10, and 20 μg/mL ATA for 16 h, expression changes of seven genes which were upregulated (p21, IL-8, TNF, and c-Jun) or downregulated (cyclinE1, AKT, and PLAU) in PCR array analysis detected by RT-PCR using specific primers as described in the text. The housekeeping gene β-actin was used as endogenous control. The figures shown were representative of three independent experiments. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as ** and *** .
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