Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
Figure 3
SBL suppresses the tBHP-induced ROS formation in PC12 cells. (a) Cultured PC12 cells were exposed to tBHP (0–300 μM) for 1 hour. The level of intracellular ROS formation was measured by fluorescence method. The results are shown in percentage of increase in ROS formation relative to the control (without tBHP). (b) Cultured PC12 cells were pretreated with SBL (3–12 μg/mL), NRR (6 and 12 μg/mL), and volatile oil (2 μg/mL) for 24 hours and then exposed to tBHP (100 μM) for 1 hour. The pretreatment of tBHQ (3 μM) was used for comparison. The results are shown in percentage of ROS formation relative to the control (with tBHP alone). Data are expressed as Mean ± SEM, where , each with triplicate samples. * and ** as compared to the group (with tBHP alone).
