Redox Mechanisms of AVS022, an Oriental Polyherbal Formula, and Its Component Herbs in Protection against Induction of Matrix Metalloproteinase-1 in UVA-Irradiated Keratinocyte HaCaT Cells

Figure 6

Inhibition of UVA-mediated catalase inactivation in HaCaT cells by the whole formula (a) and individual component herbs (b) of AVS022 and GA (c). The formaldehyde generated was determined spectrophotometrically with Purpald as a chromogen at 540 nm as described in Section 2. The statistical significance of differences between the control and irradiated cells was assessed by Student’s 𝑡-test and between UVA-irradiated and herb extracts- or GA-treated cells by one-way ANOVA followed by Dunnett’s test. ^###𝑃<0.001 compared with irradiated cells. *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001 compared with nontreated cells exposed to UVA.