Immunosuppression of the Trimellitic Anhydride-Induced Th2 Response by Novel Nonanatural Products Mixture in Mice

Figure 3

Inhibitory effect of NPM-9 in inflamed regions and splenocytes from TMA-induced allergic mice. (a) IgE levels in serum were measured by ELISA. To measure IgE, collected sera were diluted 1 : 50. (b) IL-1𝛽 was quantified by ELISA after homogenization in PBS-T using inflamed ear skin from TMA-induced BALB/c mice. Secreted (c) IL-4 and (d) IL-1𝛽 were quantified by ELISA after 72 h culture using splenocytes from TMA-induced BALB/c mice. Values are presented as mean ± SD (𝑛=4 per group). Data were analyzed by ANOVA followed by Student’s 𝑡 test. *𝑃<0.05, **𝑃<0.01, significantly different from the saline value.

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