Trans-β-Caryophyllene: An Effective Antileishmanial Compound Found in Commercial Copaiba Oil (Copaifera spp.)
Figure 10
Role of C4 oil volatile (VF) and nonvolatile (NVF) fractions and β-caryophyllene (CAR) in nitric oxide (NO) production. (a). Murine peritoneal macrophages activated or not with IFN-γ were treated with 10 μg/mL of VF, NVF, and CAR. After 48 h, culture supernatant was collected and nitrite production measured by the Griess method. Medium represents untreated and unstimulated and IFN-γ-stimulated macrophages controls. Results represent the mean ± standard error of mean (SEM) of three independent experiments in triplicates. (b). Scavenger activity of VF, NVF, and CAR. Compounds at 10 μg/mL were incubated with 1 μM of SNAP, for 6 h and nitrite production measured by the Griess method. RPMI medium was used as negative control (medium); SNAP as NO donor was incubated with rutin (an NO scavenger) as a control. Results represent the mean ± SEM of three independent experiments. .
