Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes

Figure 3

Effect of Gambisan and its major component extracts on the TG deposition and DNA contents in 3T3-L1 cells. 3T3-L1 cells were treated with various doses (0, 100, 250, and 500 μg/mL) of Gambisan or its major components (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.). After 10 days, the cellular TG contents (a) were measured with TG determination kit at 550 nm. The DNA contents in 3T3-L1 cells (b) were measured as internal control. Values are expressed as mean ± SEM of five independent experiments. *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 compared with control (expressed by t-test). EIS: Ephedra intermedia Schrenk, ALL: Atractylodes lancea DC., TSL: Thea sinensis L.

(a)

(b)